Design and Synthesize a Biomodule

Create, annotate, mutate and share your DNA building blocks

Delivered as linear dsDNA or subcloned

What is a Biomodule?

A Biomodule is a linear double-stranded DNA fragment with a well defined biological function such as CDS, terminator or gRNA.
Biomodule are the building blocks of TriLink's platform and allow you to design large and complex synthetic biological constructs by simply assembling small and well-characterized DNA parts. The main advantage behind Biomodule is the modularity concept, any Biomodule can be reused in different constructs to streamline and speed-up the design process.

What is the difference between a Biomodule and a BioBrick?

If you are wondering what the difference between biomodules and BioBricks® is, well, the very modular concept behind biomodules and BioBricks® is the same. However, TriLink's biomodules are usually longer than 25bp so that you can actually handle them in the wet lab. At the same time, a biomodule is usually shorter than 25kbp since extremely long dsDNA fragments are highly unstable in downstream applications, such as genome assembly and mutagenesis.

Tips & Tricks

Biomodules usually do not contain any restriction sites required for cloning. Restriction sites are rather usually carried by the Backbone to allow easy swapping between different plasmids.

Name

Sequence

Accepted inputs: A, C, G, T, W (weak: A, T), S (strong: C, G ), M (amino: A, C), K (keto: G, T), R (purine: A, G ), Y (pyrimidine: C, T), B (not A), D (not C), H (not G), V (not T), N (any basebase)

Labels

Upload valid FASTA, Genbank or CSV

Sequence and name fields will be overridden

Biosafety level*

Level 1

Level 2

By selecting the biosafety level corresponding to the project, customer confirms that (i) such classification is correct and accurate and (ii) that the customer has read, understood and accepted the biosafety confirmation.

I declare this DNA sequence is compliant with applicable biosecurity provisions as defined by US Select Agents and Toxins List and/or European Union Council Regulation 428/2009.*

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